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The research questions being addressed by BARN will be distributed over 6 work packages. Each work package has a WP-Coordinator. The six work packages were defined in such a way that a significant area of root biology will be covered while a maximal level of focus will be maintained. The research themes representing the centres of interest are: - root growth and architecture as the result of the interplay between cell division and cell elongation,
- the hormonal signalling involved in root growth,
- root-shoot signalling in floral transition,
- the effect of abiotic stress conditions on root development
- nutrient and water transport in roots in relation to respectively H-ATPase and aquaporin plasma membrane transporters.
The acquired biological data will be implemented in a multi-scale modelling framework that will be created in a separate work package. Such a modelling framework will be instrumental to assist in finding answers to the major biological questions of the network. In addition, it is the intention to realise an important exchange of up-to-date specialised techniques that are available in several of the contributing laboratories by defining integrated advanced technical platforms. These platforms will be available for all partners of the network and will be essential tools to answer the biological questions of this network.
WP1: Cell division and root growth – Coordinator : P1A Partners involved : P1, P4, P5, PEU1 WP1.1. Cell division in apical meristems and root growth rate WP1.2. The exit from cell division and the switch from the mitotic cycle to the endocycle in the root WP1.3. The onset of cell division in the pericycle and the initiation of new lateral roots Detailed description
WP2: Cell elongation and root growth – Coordinator : P5 Partners involved : P1, P4, P5, PEU1 WP2.1. Mapping of elongation activity along the root. WP2.2. Role of aquaporins and H+-ATPases in cell elongation WP2.3. Cell wall, cytoskeleton and elongation control WP2.4. Hormonal signalling and elongation Detailed description WP3: Hormonal signalling and root growth – Coordinator : P1D Partners involved : P1, P2, P4, P5, PEU1 WP3.1. Cell type specificity in hormone signalling WP3.2. Downstream effectors in hormone signalling Detailed description
WP4: Root-shoot interrelationship – Coordinator : P2 Partners involved : P1, P2, P3, P4 WP4.1. Root phenotype and sensitivity of flowering-time mutants WP4.2. Relationship between flowering signals of root- and leaf- origin WP4.3. SAM/RAM follow-up at floral transition Detailed description
WP5: Transport, abiotic stress and root development – Coordinator : P3/P4 Partners involved : P1, P2, P3, P4, P5 WP5.1. H+-ATPases WP5.2. Aquaporins WP5.3. Heavy metal stress WP5.4. Nutrient deficiency Detailed description
WP6: Modelling root development – Coordinator : P4C Partners involved : P1, P4, PEU1 WP6.1. Development of new imaging tools WP6.2. Design and implementation of a multi-scale modelling framework WP6.3. Systems Biology-based modelling of root elongation and branching Detailed description
INTEGRATED ADVANCED TECHNICAL PLATFORMS
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